The Effect of the Length of Time Tumour Cells spend in Suspension on their Ability to Establish Growths in Host Rats

INTRODUCTION MOST methods of studying the effects of chemotherapeutic agents on tumours in the rat involve the ability to obtain a reproducible series of tumours in similar animals at the same time. This is done by transplanting portions of a suspension of cells prepared from a tumour in a donor animal to a series of host rats of the same breed. (Lucke et al., 1952, and Cole et al., 1956). The conditions which will affect this process are many, but given a standard tumour of known reproducibility in a standard breed of animal, the main factors will be:-